Part:BBa_J119441:Design

c-Myc promoter i-Motif and G-quadruplex

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

I had to design 5' sticky ends that would allow the part to be ligated into a plasmid with either the G-quadruplex or the i-motif on the coding strand.

Source

This part comes from the human genome. It is located in the Nuclease Hypersensitive Element II of the human c-Myc oncogene promoter.